Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets.
Identifieur interne : 002104 ( Ncbi/Merge ); précédent : 002103; suivant : 002105Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets.
Auteurs : Y. Shoyama [Japon] ; X X Zhu [Japon] ; R. Nakai [Japon] ; S. Shiraishi [Japon] ; H. Kohda [Japon]Source :
- Plant cell reports [ 1432-203X ] ; 1997.
Abstract
Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets ofP. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity ofP. notoginseng. The amplification products were monomorphic for all of the plantlets ofP. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.
DOI: 10.1007/BF01092764
PubMed: 30727630
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Kohda</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Pharmaceutical Sciences, Hiroshima University, School of Medicine, Kasumi 1-2-3, 734, Minami-ku, Hiroshima, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Hiroshima University, School of Medicine, Kasumi 1-2-3, 734, Minami-ku, Hiroshima</wicri:regionArea><wicri:noRegion>Hiroshima</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A<sub>3</sub>(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets ofP. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity ofP. notoginseng. The amplification products were monomorphic for all of the plantlets ofP. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30727630</PMID><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>7</Issue><PubDate><Year>1997</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep.</ISOAbbreviation></Journal><ArticleTitle>Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets.</ArticleTitle><Pagination><MedlinePgn>450-453</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF01092764</ELocationID><Abstract><AbstractText>Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A<sub>3</sub>(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets ofP. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity ofP. notoginseng. The amplification products were monomorphic for all of the plantlets ofP. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shoyama</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Faculty of Pharmaceutical Sciences, Kyushu University, 812, Higashiku, Fukuoka, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>X X</ForeName><Initials>XX</Initials><AffiliationInfo><Affiliation>Faculty of Pharmaceutical Sciences, Kyushu University, 812, Higashiku, Fukuoka, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nakai</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Faculty of Pharmaceutical Sciences, Kyushu University, 812, Higashiku, Fukuoka, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shiraishi</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kohda</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Pharmaceutical Sciences, Hiroshima University, School of Medicine, Kasumi 1-2-3, 734, Minami-ku, Hiroshima, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Genetic homogeneity</Keyword><Keyword MajorTopicYN="N">Micropropagation</Keyword><Keyword MajorTopicYN="N">Panax notoginseng</Keyword><Keyword MajorTopicYN="N">RAPD analysis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>1996</Year><Month>04</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>1996</Year><Month>09</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>1996</Year><Month>09</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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IdType="pubmed">8820914</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Kyūshū</li><li>Préfecture de Fukuoka</li></region><settlement><li>Fukuoka</li></settlement><orgName><li>Université de Kyūshū</li></orgName></list><tree><country name="Japon"><region name="Kyūshū"><name sortKey="Shoyama, Y" sort="Shoyama, Y" uniqKey="Shoyama Y" first="Y" last="Shoyama">Y. Shoyama</name></region><name sortKey="Kohda, H" sort="Kohda, H" uniqKey="Kohda H" first="H" last="Kohda">H. Kohda</name><name sortKey="Nakai, R" sort="Nakai, R" uniqKey="Nakai R" first="R" last="Nakai">R. Nakai</name><name sortKey="Shiraishi, S" sort="Shiraishi, S" uniqKey="Shiraishi S" first="S" last="Shiraishi">S. Shiraishi</name><name sortKey="Zhu, X X" sort="Zhu, X X" uniqKey="Zhu X" first="X X" last="Zhu">X X Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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